标题: BloodVitals SPO2: How I Streamlined My Daily Health Checks [打印本页] 作者: LeilaRaymo 时间: 2025-9-14 01:02 标题: BloodVitals SPO2: How I Streamlined My Daily Health Checks
Important cell populations reside inside tissues and are usually not accessed by traditional blood draws used to monitor the immune system. To deal with this problem at an important barrier tissue, the pores and skin, we created a microneedle-primarily based know-how for longitudinal sampling of cells and interstitial fluid, enabling minimally invasive parallel monitoring of immune responses. Solid microneedle projections have been coated by a cross-linked biocompatible polymer, which swells upon pores and skin insertion, forming a porous matrix for native leukocyte infiltration. By embedding molecular adjuvants and specific antigens encapsulated in nanocapsules within the hydrogel coating, antigen-specific lymphocytes could be enriched within the recovered cell inhabitants, permitting for subsequent detailed phenotypic and practical evaluation. We reveal this strategy in mice immunized with a model protein antigen or contaminated in the skin with vaccinia virus. After vaccination or infection, sampling microneedles allowed tissue-resident reminiscence T cells (TRMs) to be longitudinally monitored in the skin for many months, throughout which time the antigen-particular T cell inhabitants in systemic circulation contracted to low or undetectable counts. Sampling microneedles did not change the immune standing of naïve or antigen-uncovered animals. We also validated the ability of cell sampling using human skin samples. This strategy may be helpful in vaccines and immunotherapies to temporally query TRM populations or as a diagnostic platform to pattern for biomarkers in chronic inflammatory and autoimmune disorders, permitting data beforehand accessible solely through invasive biopsies to be obtained in a minimally invasive manner from the pores and skin or different mucosal tissues.
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